In this study, we explored traditional prognostic factors and a panel of tumor markers not used in routine diagnosis, such as NHERF1, VEGFR1, HIF-1α and TWIST1, that have been respectively related to breast cancer progression [17-19], aggressiveness [21,22], hypoxic response [29,33] and cell invasion/metastasis [24,34], assessing if they are differentially expressed in tumors scored as grade 2, in order to best characterize them. The gene discussed is NHERF1; the disease is breast cancer.