All breast cancers showed NHERF1 protein localized in the cytoplasm of tumor cells and 31% of overexpressing cytoplasmic NHERF1 tumors exhibited a significant association with tumor grade 3 (p = 0.035), negative PR status (p = 0.008), high MIB1 (p = 0.033), positive HER2 status (p = 0.036) and with moderate NPI (p = 0.029). This evidence concerns the gene NHERF1 and breast cancer.