Following previous PCR-based observations that Hiwi is expressed in sarcomas [15] and that its expression correlates with prognosis [15], we examined Hiwi protein levels via immunohistochemistry (IHC) in a large primary human sarcoma tissue microarray (TMA) composed of numerous soft-tissue sarcomas (previously described by us [18]). Here, PIWIL1 is linked to sarcoma.