Quantitation of signals from protein analysis by western blot hybridization shows that ratio of peIF-2α to eIF- 2α decreases from 3- to 42-fold in 11 of 15 osteosarcoma tissues analyzed, demonstrating an increased phosphorylation of eIF-2α in normal compared to tumor (Figure 6D). The gene discussed is EIF2A; the disease is neoplasm.