We examined various glioma cells for MSI1 protein expression by immunoblotting, and found it in human glioblastoma cell lines (KNS42 and U251MG), low-passage glioblastoma cell lines obtained from patients (GM1600, GM1605, and GM97), and a human medulloblastoma cell line (Daoy) (Fig. 1A; compare with the level in hNSCs). The gene discussed is MSI1; the disease is central nervous system cancer.