MITF and melanoma: To ascertain whether decreased Mitf expression could fully explain the depigmentation effects of pyridinyl imidazole compounds, we transiently transfected B16-F0 melanoma cells with a plasmid encoding for Mitf cDNA (pCAAG-mi-S) or a control construct carrying Mitf cDNA in antisense orientation (pCAAG-mi-AS).