To investigate whether circulating Hsp20 is increased in animals subjected to myocardial ischemia/reperfusion (I/R), 12-weeks old mice (FVB/N) underwent in vivo 30-min myocardial ischemia, via coronary artery occlusion, followed by 24-h reperfusion. Here, HSPB6 is linked to myocardial ischemia.