We sought to: 1) determine which GR alternative 5′ exons are employed in the adult human DLPFC, 2) determine whether expression levels of these alternative GR mRNA transcript variants are altered in schizophrenia and/or bipolar disorder cases relative to controls; and 3) explore whether NR3C1 polymorphisms may be associated with GR mRNA transcript and GRα protein isoform expression levels in human brain. Here, NR3C1 is linked to bipolar disorder.