AMOT and in situ carcinoma: Amot expression evaluated by Western blot from protein extracts of mammary glands of BALB-neuT transgenic mice bearing foci of hyperplasia (week 6), in situ carcinomas (week 10), or microscopic invasive cancer (week 22), and from autochthonous carcinomas of progressive size (from 2 to 10 mm mean diameter) (Fig. 1a), showed that the level of Amot protein increases from pre-neoplastic lesions to full-fledged lobular carcinoma (Fig. 1a).