In our study, we performed an automated image analysis on a set of 10 IHC markers, including the conventional ER, PR, HER2, and Ki67 along with less investigated androgen receptor (AR), BCL2, HIF-1α, SATB1, p53 and p16 on tissue microarrays (TMAs) of 109 patients with ductal carcinoma of the breast. The gene discussed is ESR1; the disease is breast ductal adenocarcinoma.