The neuroblastoma derived cell line, SK-N-SH, have been used previously to study the heterologous expression of mutant rod opsins [14–16], therefore, SK-N-SH cells were transfected with pEGFP-N1-Rho, pEGFP-N1-Rho(P23H), or pEGFP-N1-Rho(c.614–622del), encoding wild-type (WT) opsin-GFP, P23H opsin GFP, and Y206-F208del opsin-GFP, respectively. This evidence concerns the gene RHO and neuroblastoma.