The effect of IL-1β and IP6 on the expression of mRNA of MMP-1, MMP-2, MMP-3, MMP-9, MMP-10, and MMP-13 and that of their tissue inhibitors TIMP-1 and TIMP-2 in colon cancer cells using real-time QRT-PCR assay was determined. The gene discussed is TIMP1; the disease is malignant colon neoplasm.