After 6 weeks on a HF diet, DIO rats receiving leptin (i.p.; 4.94 nmol/kg) no longer significantly inhibited food intake even in the presence of endogenous release of CCK by a preload meal (LF: p<0.01; DR: p<0.01; DIO: p>0.05; Fig. 4D). The gene discussed is LEP; the disease is hydrops fetalis.