MTOR and malignant colon neoplasm: LS174T (PI3KCA mutation on exon 20), DLD-1 (PI3KCA mutation on exon 9) and SW480 (PI3KCA wild type) colon cancer cells were treated with increasing concentrations of rapamycin, PP242 [19], a specific mTOR inhibitor, or NVP-BEZ235 [20], a dual PI3K/mTOR inhibitor for six hours.