Rcan1-4 mRNA expression in the same brain ischemia model was determined by real time qRT-PCR, together with the mRNA expression levels of the proinflammatory cytokines IL-1β, TNFα and interleukin 6 (IL-6) and the proinflammatory gene cyclo-oxygenase 2 (Cox-2). This evidence concerns the gene TNF and brain ischemia.