YAP1 and pancreatic neoplasm: As shown in Figure 3, at lower cell densities (20–70%), the YAP1 protein was present in both the cytoplasm and nucleus in the two pancreatic cancer cell lines, BxPC-3, and PANC-1, whereas in the immortalized normal pancreatic ductal epithelial cell line, HPDE6, YAP1 was not detectable in the cytosolic fraction.