However, the constellation of cardiovascular outflow tract, great vessel, pharyngeal organ, and craniofacial defects that collectively typify DiGeorge syndrome had not been previously recognized in Tie2Cre/Nrp1 mutants, and could not have been seen (because of early lethality) in global Nrp1 mutants. This evidence concerns the gene NRP1 and 22q11.2 deletion syndrome.