To further determine at which level the absence of N1 and N2 on T cells affects secretion of IFNγ in the dLN of L. major-infected mice, mRNA levels of IFNγ and T-bet, the major transcription factor of Th1 cells, were measured ex vivo on FACS sorted CD4+ T cells 3 weeks after infection. Here, CD4 is linked to infection.