When our cohort of seminomas was examined on the basis of the same methods and criteria,21 CAM5.2 reactivity (15/87 cases, 17.2%) was significantly correlated with widely scattered nuclear reactivity for DNMT3B (P = 0.0028), whereas histological atypia (P = 0.321) and expression of CD30 (P = 0.0653) and CD117 (P = 0.0814) did not show such significant correlations. This evidence concerns the gene TNFRSF8 and seminoma.