However, as NO generated by NOS has been implicated in PD, the role of NOS-sGC was investigated via functional assays in Drosophila. We tested whether the Drosophila NO signal components dNOS and sGC are indeed involved in FoxO and DG2-mediated DA neurodegeneration in Drosophila (Fig. 8). This evidence concerns the gene NOS1 and Parkinson disease.