In order to evaluate for the possibility of C3(H2O) being generated in hyperglycemic conditions, we tested whether C3 in 3 or 17 mmol/L glucose would be cleaved by the presence of purified factors H and I. C3 remained intact in conditions that efficiently cleaved C3b to iC3b suggesting that hyperglycemia was not generating C3(H2O) (Figure 1D). Here, C3 is linked to Hyperglycemia.