ASCL2 and neoplasm: Furthermore, Ascl2 expression in the tumor tissue from shRNA-Ascl2/HT-29 or shRNA-Ascl2/LS174T cells was significantly lower than that of shRNA-Ctr/HT-29 or shRNA-Ctr/LS174T cells, as determined by real-time PCR and western blot analysis (Figure 3D and 3E).