MAVS and infection: Figure 3a shows MAVS protein in sections of uninfected cells stained with rabbit anti-MAVS antibody followed by secondary anti-rabbit antibody conjugated with 15 nm gold (black arrow). Figure 3b shows His6-NS1 in sections of cells stained with 5 nm gold-Ni-NTA 12 hours after rA2-His6-NS1 infection (red arrow). Figure 3c and d show cell sections 12 and 24 h post-infection respectively, stained with anti-MAVS antibody (15 nm gold) followed by Ni-NTA (5 nm gold) antibodies. These results demonstrate that His6-NS1 colocalizes with MAVS on mitochondria (M).