HEp-2 cells were mock-infected or infected at an MOI of 1 with either rA2or rA2 ΔNS1 orrA2 ΔNS2 and at 24 h post-infection (p.i.), total cell lysates were immunoprecipitated with anti-MAVS antibody and analyzed by western blotting with anti-NS1 antiserum. The gene discussed is MAVS; the disease is infection.