The virulent strain wMelPop-CLA activates a wide range of immune processes in A. aegypti, including the Toll and Imd signaling pathways, anti-microbial peptide synthesis, melanization, RNA interference and opsonisation [9], [10] and the somatic infection of An. gambiae by wMelPop caused an increase in expression of opsonisation genes [11]. Here, TLR4 is linked to infection.