RNA was collected at intervals between 0 and 12 h of infection and analyzed by quantitative reverse transcription PCR (qRT-PCR) using primers specific to lytic transcripts representing each kinetic class: IE (ICP27/UL54), E (DNA polymerase/UL30), leaky-late/γ1 (VP16/UL48) and true-late/γ2 (tegument phosphoprotein ICP1-2/UL36). Here, RPL7 is linked to infection.