In all these cases, MAML1 was coamplified with FLT4. To confirm that MAML1 may be a “driver” gene of the 5q35.3 amplicon, we have assessed its mRNA by qRT-PCR (3 AS cases with MAML1 amplification, 17 AS cases without MAML1 amplification, and 2 other vascular tumors) and protein expression by Western blotting (one case with MAML1 amplification, four AS cases without MAML1 amplification and the SKBR3 cell line). Here, MAML1 is linked to vascular neoplasm.