UBC and ovarian carcinoma: In addition to the B16F0 cell line, three additional high PRL-3-expressing cell lines were identified: HCT116, a human colorectal cancer cell line; HCT116-luc2, a HCT116 cell line that was established by transducing lentivirus containing the luciferase 2 gene (luc2) under the control of human ubiquitin C promoter; and A2780, a human ovarian cancer cell line (Fig. 3A lanes 1-3).