Significant granzyme B secretion by 14.G2a-28ζ gene-modified vs non-transduced T cells was found in response to both GD2int (TC-71, TC-32) and GD2low (VH-64, A4573, Cado-ES-1) cell lines, except for the lowest GD2-expressing Ewing sarcoma cell lines WE-68, TTC-466 where responses did not reach statistical significance. The gene discussed is GZMB; the disease is Ewing sarcoma.