To further examine the function of DPIV, APN and/or DPIV-/APN-like peptidases in ischemia-dependent neurodegeneration, we have studied these target peptidases in an in vivo model of transient, unilateral cerebral ischemia due to endothelin-induced occlusion of the middle cerebral artery (eMCAO). This evidence concerns the gene ANPEP and brain ischemia.