CCNK and cyst: In our study, Western blot analysis of different portions of the cyst extracts confirmed that cyclin K and the hyperphosphorylated form of RNAP II (IIo), detected by the H5 antibody, were both located in the nuclei of 14-h incubated embryos (Fig. 3C), but immunofluorescence staining of nuclei showed that the cyclin K staining degree were not proportional to the staining of IIo (Fig. 3D), and IIo was not detected in cyclin K immunoprecipitates (IPs) from 14-h incubated embryo extracts (Fig. 3E).