CALR and systemic lupus erythematosus: Using the segregation algorithm developed for the pilot cohort, we observed in the validation cohort that 47% (61/129) of the SLE samples showed a Sm/RNP cluster phenotype, 51% (66/129) showed a Ro/La cluster phenotype and 2% (2/129) displayed no significant autoantibody responses to any of the antigens tested (Fig. 2).