As we had previously identified SFRP4 to be aberrantly expressed at the RNA level in a large transcriptional profiling experiment of ovarian cancer patients (unpublished data), here we investigate for the first time SFRP4 RNA and protein expression in 725 patients using reverse transcription quantitative polymerase chain reaction (RT-qPCR), Western-blot, immunohistochemistry (IHC) and capture enzyme-linked immunosorbent assay (ELISA) in primary cultures, ovarian cell lines, ascites, tissue and plasma. This evidence concerns the gene SFRP4 and ovarian carcinoma.