Furthermore, a possible functional role of the MCV T antigens on AKT activation in MCC was tested by silencing T antigen expression in four MCV positive MCC cell lines by infection with a lentiviral shRNA construct targeting Large as well as small T antigen (LT and sT) mRNA; LT and sT mRNA are derived by alternative splicing from the same genomic locus and share common sequences in exon 1 [3]. Here, AKT1 is linked to infection.