Here we show that suppression of p110α expression in NCI-H460 cells markedly reduced in vitro anchorage-dependent and in vivo tumour growth of cells subcutaneously injected into immunodeficient mice (n = 6/group) (Figure 6C and D, respectively), indicating that PI3K activation plays a significant role in the malignant behaviour of NSCLC cells. This evidence concerns the gene PIK3CA and neoplasm.