IGFBP3 and type 1 diabetes mellitus: Western blot analysis of IGFBP3 suggests that the apparent increase in IGFBP3 levels in mothers with T1DM may be due to increased proteolysis of IGFBP3, though the rise in IGFBP3 in fetal blood appears to be genuine, since there was no apparent difference in fetal IGFBP-3 proteolysis as determined by western immunoblot analysis.