The present study logically extends our previous findings [11]–[13] to systematically investigate the role of B-cell lymphoma 2 interacting mediator of cell death (Bim), p53 upregulated modulator of apoptosis (PUMA), Bcl-xL, and Bcl-2 proteins in regulation of BITC-induced apoptosis using MCF-7 (breast), MDA-MB-231 (breast), and HCT-116 (colon) human cancer cells as a model. This evidence concerns the gene BCL2 and cancer.