PLS1 and listeriosis: To determine whether the IFN-γ benefit conferred or mediated by our top hit genes is specific to F. tularensis infection or functions more generally in response to intracellular pathogens, we investigated the effect of 8 of the top hit genes from our screen (TNFRSF9, SERPINI1, SERPINA7, HLA-DRB1, ATG5, ATG16L1, PLEK2 and PLS1) on Listeria monocytogenes infection in THP-1 cells stably transduced with shRNAs targeting these genes.