In this study, we demonstrate that BTG1, a recurrent target in pediatric ALL, is affected by gene truncating deletions, which occur predominantly in two cytogenetic subgroups of pediatric BCP-ALL, i.e., the ETV6-RUNX1 positive (19%) and BCR-ABL1 positive (26%) subgroups. This evidence concerns the gene BTG1 and acute lymphoblastic leukemia.