GNAQ and infection: nematophilum strain did not alter levels of ACh release suggesting that changes in ACh release are in response to infection by M. nematophilum. Reduction-of-function mutations in EGL-30 (Gαq) are resistant to aldicarb ([29] and Figure 1B) and infection of egl-30(ad805) did not result in an increase in ACh release (Figure 1B) indicating that EGL-30 (Gαq) signaling is required to increase ACh release and alter locomotion behavior in response to infection.