CAV1 and breast cancer: Western blotting and immunofluorescence for Cav-1 protein and qRT-PCR for Cav-1 mRNA revealed that tumor cells themselves can express Cav-1 and the claudin-low mammary tumor cell lines (RJ348 and RM11A) expressed higher levels of Cav-1 protein and mRNA than the epithelial tumor cell lines (Figure 4 and Table 1).