Analysis of a number human ovarian cancer cell lines showed that MAP2K4 expression is not detectable in 3 cell lines (SHOV3ip.1, SKOV-3 and HEY-A8) known to be metastatic in vivo while other members of the MAP2K4 pathway are intact including MEKK1, MKK7, JNK and c-JUN. This evidence concerns the gene JUN and ovarian carcinoma.