PAX2 and cyst: In this study, we found that the cyst number in the Pax2 overexpressing cell line increased by ∼2.5-fold (P<0.05) relative to the control cell line, which expresses only the puromycin selection marker (5′Δ5N-Pac) (Figure 2C), indicating that the overexpressed Pax2 can increase the cyst formation (Figure S1D).