The status of CD4 molecules, the primary receptors of HIV-1, was important to assess in ankyrin-expressing cells prior to HIV-1 infection, in order to ensure that SupT1/Myr+AnkGAG1D4-GFP and SupT1/Myr0AnkGAG1D4-GFP cells could serve as host cells for testing the functionality of AnkGAG1D4 as antiviral agent. This evidence concerns the gene CD4 and HIV-1 infection.