To determine whether TFAs deducted from PTEN-inducible MEF reflect PTEN functional status in vivo, we examined the TFAs, based on the gene expression datasets we have in hand, of three well-established murine prostate cancer models, i.e. the Pten null [29], the mAKT1[24], and the hi-c-Myc[30] models (Figure 3A), and then categorized the TFs into subgroups according to alterations of their activities in these mouse models (Figure 3B). The gene discussed is PTEN; the disease is prostate cancer.