In order to study the TBM etiology, an immunological evaluation was performed, consisting of anti-neutrophil cytoplasmic antibody (ANCA) directed to proteinase 3 (PR3), ANCA myeloperoxidase (MPO), antibody anti-basal membrane, anti-centromere, anti-Jo, anti-RNP (ribonucleoprotein), anti-scleroderma (anti-Slc70), anti-Smith (Anti-Sm), Anti-SSa, Anti-SSb and anti-nuclear antibody. The gene discussed is MPO; the disease is scleroderma.