The vast majority of tumours with strong SATB2 intensity had a nuclear fraction of >75% (Figures 1G–H) and only a few had a nuclear fraction of 50–75% (Figure 1F), hence the low number (n=4) of tumours denoted as having NS=9 (Supplementary Figure 1A), whereas an NS of 8 (n=104) would correspond to moderate staining in >75% of tumour cells (Figure 1E). The gene discussed is SATB2; the disease is neoplasm.