As CRT analysis suggested an optimal cutoff point at NS >9 to determine the impact of SATB2 expression of CSS and OS (Supplementary Figures 1D-E), three categories of SATB2 expression were constructed for comparison of variable distribution: SATB2-negative tumours (NS=0), corresponding to the diagnostic threshold, an intermediate category (NS 1–9) and SATB2 high tumours (NS>9), corresponding to the prognostic threshold derived from CRT analysis. This evidence concerns the gene SATB2 and neoplasm.