To study the release of the two chemokines CXCL9 and CXCL10 from breast cancer cells we investigated regulation of protein secretion by supplying the inflammatory cytokines IFN-γ and TNF-α, which are both present in breast cancer tissues and known to induce CXCR3 ligands in other cell types [6,28,29]. Here, IFNG is linked to breast cancer.