In contrast, the use of the BCG::RD1-ESAT-6Δ84–95 strain that expresses and secretes truncated ESAT-6 (Figure S4B), showed no switch of the FRET ratio to higher values throughout the time course, indicating the absence of bacteria in the cytosol during the measured infection period (Figures 3C, 3F). This evidence concerns the gene PDE6B and infection.