When conditioned medium from metastatic human breast cancer cells, MDA-MB-231, was added to human osteoblasts (hFOB1.19) or murine (MC3T3-E1) or primary osteoblasts, the osteoblasts increased their secretion of interleukin-6 (IL-6), interleukin-8 (IL-8), and monocyte chemoattractant protein-1 (MCP-1). The gene discussed is CCL2; the disease is breast cancer.