Immunohistochemical analysis of subcutaneous tumour formed after injection of UMSCC81B-GR3 cells into nude mice showed that E-cadherin(−)/vimentin(+) tumour cells that are less cohesive and exhibit a poorly-differentiated morphology proliferated at the invasion front of the tumour, but were almost absent in parental UMSCC81B tumour (Figure 1A). This evidence concerns the gene CDH1 and neoplasm.