We suggested the apoptosis or sensitivity to gemcitabine induced by blocking PI3K/AKT might be ascribed to inhibition of NF-κB activity at least in part, the other signaling pathway may take part in the downstream signaling events of the AKT activity regulation, NF-κB may not be the main mechanisms of apoptosis regulation in some pancreatic cancer cell lines. The gene discussed is AKT1; the disease is familial pancreatic carcinoma.